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. 2016 May 24;24(7):1237–1246. doi: 10.1038/mt.2016.70

Figure 2.

Figure 2

Transduction of primary cells by VSV-G and cocal LV.(a) Transduction efficiency of nonhuman primate (NHP) CD34+ cells (n = 3) exposed to lentiviral vectors (LVs) (PGK.eGFP, generated with standard protocol) using two doses of vector of 6 hours each at multiplicities of infection (MOI) of five. Mean percentage of eGFP+ cells was determined at 10 days post-transduction. PGK, phosphoglycerate kinase (1 promoter human); eGFP, enhanced green fluorescent protein. (b) Mean transduction efficiency of human CD34+ cells (n = 2) exposed to LVs at an MOI of 5. The percentage of eGFP+ cells was determined 6 days post transduction. (c) Human CD34+ cells from one of the donor described in b were plated for colony-forming cells (CFCs). The fraction of progenitor cells for different lineages was enumerated and compared between mock, VSV-G or cocal LV transduction. E, erythroid; M, monocyte; GM, granulocyte/macrophage; GEMM, granulocyte/erythrocyte/macrophage/megakaryocyte. (d) Transduction efficiency in NHP peripheral blood from two donors enriched for CD4+, activated for 3 days and exposed to LVs at MOI of 5. The percentage of eGFP+ cells was determined at 3 days post transduction from reactions run in duplicates. (e) Transduction efficiency in human CD4+ cells exposed to two different LV preparations (LV1 and LV2) at MOI of 1. The percentage of eGFP+ cells was determined at 3 days post transduction. In all experiments, error bars show SE of the mean.