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. 2016 May 24;24(7):1237–1246. doi: 10.1038/mt.2016.70

Figure 3.

Figure 3

sLDLR inhibits VSV-G and cocal LV transduction. (a) Dose-dependent inhibition of vesicular stomatitis virus envelope glycoprotein (VSV-G) and cocal pseudotyped LV (cocal LV) transduction of HT1080 cells by human soluble LDLR (sLDLR). 100x concentrated foamy virus vector (FV), VSV-G LV and cocal-LV (all PGK.eGFP, generated with standard transfection protocol) were preincubated with different concentrations of sLDLR (in μg/ml) and used to transduce HT1080 cells. The percentage of eGFP+ cells was determined at 3 days post transduction from reactions run in triplicates, and results show the percentage of infectivity in comparison to untreated cells. (b) Transduction of nonhuman primate (NHP) CD34+ cells by FV, VSV-G LV and cocal LV in the presence (10 μg/ml) or absence of sLDLR. Results show mean % infectivity taken from two time points (2 and 6 days post transduction) relative to untreated cells. In all experiments, error bars show SE of the mean.