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. 2016 May 24;24(7):1237–1246. doi: 10.1038/mt.2016.70

Figure 7.

Figure 7

Adaptation of cocal producer cell lines to growth in suspension/serum free media. (a) Growth rate of 293T-cells, eGFP 2–12 and C4 1–9 producer cells adapted to growth in suspension/serum free media. Cells were passaged every 3–4 days, and viable count was determined by trypan blue staining. (b) Unconcentrated LV titer (flow cytometry, bar graph) and viable cell count (line graph) measured in 2–12 eGFP LV producer cells grown in suspension following induction with sodium butyrate (NaBu) (T0). (c) Unconcentrated LV titer quantitative PCR (qPCR, bar graph) and viable cell count (line graph) measured in C4 1–9 LV producer cells grown in suspension following induction with NaBu (T0).