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Flow cytometry assay for studying specific binding of pRNA-3WJ nanoparticles on prostate cancer cells. (a) Illustration of various design of conjugating prostate-specific membrane antigen (PSMA) binding A9g aptamer onto the pRNA-3WJ core. Alexa647 labelled A9g-3WJ RNA nanoparticles were incubated with (b) PSMA+ LNCaP-FGC cells and (c) PSMA- PC-3 cells. Nanoparticles indicated positive binding of nanoparticles to LNCaP cells while avoiding nonspecific binding to PC-3 cells.
