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. 2016 Jul 19;24(7):1267–1277. doi: 10.1038/mt.2016.85

Figure 6.

Figure 6

Caspase III signaling assay for apoptosis effects of pRNA-3WJ nanoparticles harboring prostate-specific membrane antigen binding aptamer and antioncogenic miRNA LNA. RNA nanoparticles with anti-miR21 and -miR17 LNA were incubated with (a) LNCaP and (b) PC-3 cells cells for 24 hours. Caspase III signaling was assayed by fluorescent reporter with peak fluorescent of 440 nm as a reporter of cell apoptosis as a result of RNA nanoparticle delivery of anti-miRNA sequences. 5 μmol/l Camptothecin was used as a positive control and benchmark of apoptosis (**P < 0.01, ***P < 0.001).