Figure 2.

Pyruvate kinase deficiency phenotype correction in secondary transplanted mice. (a) Brilliant Cresyl blue staining of blood smears from nontransplanted mice and secondary (2nd coRPK) recipients to identify reticulocyte population (in blue). (b) Flow cytometry analysis of reticulocyte levels in peripheral blood. (c) RBCs and (d) reticulocyte percentage in secondary transplanted mice expressing the coRPK transgene (scratched bar, n = 4), in healthy mice (black bar, n = 3) and in anemic control mice (gray bar, n = 3). Data are represented the average ± standard error of the mean and were analyzed by nonparametric two-tailed Mann-Whitney test.