Figure 3.

Normalization of the erythroid differentiation pattern in genetically corrected mice. (a) Percentages of the different erythroid subpopulation in bone marrow and spleen at 140 days after transplant. (b) Representative dot plots of the flow cytometry strategy used. The expression intensity of the CD71 and Ter119 markers allows for identifying four erythroid subpopulations: population I: early proerythroblasts (Ter119^med CD71^high), population II: basophilic erythroblasts (Ter119^high CD71^high), population III: late basophilic and polychromatophilic erythroblasts (Ter119^high CD71^med), and population IV: orthochromatophilic erythroblasts, reticulocytes and mature erythroid cells (Ter119^high CD71^low). (c) Plasma Epo levels measured by enzyme-linked immunosorbent assay (ELISA) in nontransplanted and transplanted mice. Dots represent values of individual mice. Lines represent average ± standard error of the mean and were analyzed by nonparametric Kruskal-Wallis test. Healthy, nontransplanted control mice; PKD, nontransplanted PKD mice; EGFP, PKD mice expressing the EGFP transgene; coRPK, PKD mice expressing the therapeutic transgene. PKD, pyruvate kinase deficiency.