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. 2016 Oct 5;3(5):ENEURO.0174-16.2016. doi: 10.1523/ENEURO.0174-16.2016

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Copyright © 2016 Yuan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 10. — Detection of prepro-CART (pCART) and prepro-MCH (pMCH) mRNAs using chromogenic ISH. A, pCART hybridization signals (brown DAB; bright-field optics) were strong in select hypothalamic nuclei. B, Throughout the nodose ganglion of fed mice, pCART signals of varying intensity were observed in many cell profiles. C, The nodose ganglion of fasted mice also contained pCART hybridization signals. D, Details of the hybridization signal in the nodose ganglion of one fasted mouse. Please note representative cell profiles without signal (*), or with low (l), medium (m), and high (h) signals. E, Hybridization signals for pMCH were very strong in neurons of the lateral hypothalamus. F, G, In contrast to the hypothalamus, pMCH signals were not observed in the nodose ganglia of fed and fasted mice. H, Details of the nodose ganglion of one fasted mouse showing several neuronal profiles completely devoid of signals (*). Tissue was counterstained with hematoxylin. 3V, third ventricle; Arc, arcuate nucleus; DMH, dorsomedial hypothalamus; LHA, lateral hypothalamus; NG, nodose ganglion; VMH, ventromedial hypothalamus; ZI, zona incerta. Scale bars, 500 μm in A and E; 50 μm in B, C, F, and G; 20 μm in D and H.