Figure 11.
Absence of MCH staining in the vagal afferents of metabolically challenged Nav1.8-Cre-ChR2-YFP mice. A, An antiserum against prepro-MCH (pMCH) labeled perikarya (Alexa Fluor 594) in the lateral hypothalamus (LHA). B, PMCH was undetectable in the nodose ganglion (NG) of fasted Nav1.8-Cre-ChR2-YFP mice. C, D, An antiserum against the MCH peptide labeled very few axons (arrowhead) in the medial NTS (SolM). MCH immunoreactivity was never observed in the YFP-labeled fibers of vagal origin. Arrowheads point to one MCH-positive axon. sol, solitary tract. Scale bars, 40 μm in A and B; 10 μm in C.