Figure 12.

CART(55–102) immunolabeling of peripheral ChR2-YFP–labeled fibers. A, ChR2-YFP fibers contained within the cervical vagus nerve. CART was not detected in the vagus nerve. We obtained similar results in the cervical and subdiaphragmatic nerves on both sides. B, In whole mounts of the gastric muscularis, numerous GFP-positive axons and specialized endings can be observed. The leafy structure may correspond to an intraganglionic laminar ending (arrowhead) of vagal origin. CART immunoreactivity was abundant in large varicose fibers and, to a lesser extent, the cell body of enteric neurons (EN). Nonetheless, CART immunoreactivity was not found in GFP-stained fibers. IGLE, intraganglionic laminar ending; EN, enteric neuron. Scale bar, 10 μm in A and B.