Table 1.
Information relative to the primary and secondary antisera used in the study.
| Antibody | Manufacturer | Catalog no. | Lot no. | Host | Working dilution | Immunogen |
|---|---|---|---|---|---|---|
| Primary antibody | ||||||
| Peripherin | EMD Millipore | AB1530 | 2446692 | Rabbit | 1:500 | trp-E-peripherin fusion protein containing all but the four N-terminal amino acids of rat peripherin |
| CART(55–102) | Phoenix Pharmaceuticals | H-003-62 | 01251-10; 01251-6 | Rabbit | 1:800–1:1000 | Ile-Pro-Ile-Tyr-Glu-Lys-Lys-Tyr -Gly-Gln-Val-Pro-Met-Cys-Asp-Ala-Gly-Glu-Gln-Cys-Ala-Val-Arg-Lys-Gly-Ala-Arg-Ile-Gly-Lys-Leu-Cys-Asp-Cys-Pro-Arg-Gly-Thr-Ser-Cys-Asn-Ser-Phe-Leu-Leu-Lys-Cys-Leu [disulfide bonds between Cys1-Cys3,Cys2-Cys5,Cys4-Cys6] |
| CART 1–39 | Phoenix Pharmaceuticals | H-003-63 | 01102 | Rabbit | 1:800–1:1000 | pGlu-Glu-Asp-Ala-Glu-Leu-Gln-Pro-Arg-Ala-Leu-Asp-Ile-Tyr-Ser-Ala-Val-Asp-Asp-Ala-Ser-His-Glu-Lys-Glu-Leu-Pro-Arg-Arg-Gln-Leu-Arg-Ala-Pro-Gly-Ala-Val-Leu-Gln |
| MCH | Phoenix Pharmaceuticals | H-070-47 | 01629-3 | Rabbit | 1:1000 | Asp-Phe-Asp-Met-Leu-Arg-Cys-Met-Leu-Gly-Arg-Val-Tyr-Arg-Pro-Cys-Trp- Gln-Val |
| TH | Abcam | ab101853 | GR120879-17 | Goat | 1:1000 | Synthetic peptide corresponding to human TH(30–100) (N terminal) |
| ProMCH | Santa Cruz Biotechnology | sc-14509 | B2415 | Goat | 1:100–1:1000 | 20-amino-acid peptide near the C-terminus of pro-MCH precursor of human origin |
| GFP | Aves Laboratory | GFP-10120 | GFP697986 | Chicken | 1:1000 | GFP emulsified in Freund’s adjuvant |
| Secondary antibody | ||||||
| Anti-rabbit Alexa Fluor 594 | Life Technologies | A21207 | 1256153 | Donkey | ||
| Anti-goat Alexa Fluor 594 | Life Technologies | A11058 | 1608643 | Donkey | ||
| Anti-chicken Alexa Fluor 488 | Life Technologies | A11039 | 1356650 | Goat |