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. 2016 Oct 5;3(5):ENEURO.0177-16.2016. doi: 10.1523/ENEURO.0177-16.2016

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Copyright © 2016 Bocchio et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 7. — 5-HT inhibits pc-nNOS neurons. A, Representative cell-attached recording from a pc-nNOS neuron (voltage-clamp mode) inhibited by bath application of 5-HT (50 μm). In this cell, 5-HT did not trigger burst firing. B, Representative cell-attached recording from a pc-nNOS neuron (voltage-clamp mode) in which bath application of 5-HT elicited a reduction in both firing rate and burst firing. Insets, Magnified examples of tonic firing in control conditions and burst firing upon bath application of 5-HT (50 μm). C, Significant decrease in firing rate promoted by 5-HT (from 3.6 ± 1.6 Hz to 1.6 ± 0.4 Hz; p < 0.0001, paired t test; n = 18). D, Significant increase in firing irregularity (measured by the CV of the ISI: from 0.5 ± 0.06 to 2.9 ± 0.4; p < 0.0001, paired t test; n = 18) caused by 5-HT. E, 5-HT application enhances the burstiness of pc-nNOS neurons: the peak of the ISI histogram (in Log scale) shifts to the left (n = 18). F, 5-HT triggered spike bursts in only 7 of 18 pc-nNOS neurons. The remaining neurons displayed a reduction in firing rate only upon 5-HT application. G, In four cells displaying bursts upon 5-HT application, 5-HT was reapplied in the presence of synaptic blockers (10 μm NBQX, 50 μm D-APV, and 10 μm SR95531). In these conditions, 5-HT still triggered bursting (the peak of the Log ISI histogram shifted to the left), suggesting that synaptic inputs are not necessary for bursting activity. ****p < 0.0001. Data are presented as the mean ± SEM.