Table 2. Chemical Shifts of Peptides [15N]1a and [15N]1b.
| δ
F20 |
δ G33 |
||||
|---|---|---|---|---|---|
| crosspeak | 1H | 15N | 1H | 15N | species |
| 1 | 8.32 | 122.3 | A monomer | ||
| 2 | 8.56 | 121.3 | A4 homotetramer | ||
| 3 | 8.39 | 112.5 | B monomer | ||
| 4 | 9.33 | 115.8 | B4 homotetramer | ||
| 5 | 8.81 | 124.9 | A2B2 heterotetramer | ||
| 6 | 9.03 | 116.2 | A2B2 heterotetramer | ||
| 7 | 8.69 | 125.7 | A3B1 heterotetramer | ||
| 8 | 8.66 | 121.1 | A3B1 heterotetramer | ||
| 9 | 8.60 | 119.3 | A3B1 heterotetramer | ||
| 10 | 7.94 | 112.8 | A3B1 heterotetramer | ||
| 11 | 8.92 | 120.9 | A1B3 heterotetramer | ||
| 12 | 8.74 | 116.1 | A1B3 heterotetramer | ||
| 13 | 9.25 | 115.8 | A1B3 heterotetramer | ||
| 14 | 9.17 | 115.1 | A1B3 heterotetramer | ||
a
1H,15N HSQC spectrum was recorded for the 1:1 mixture at 8.0 mM in 9:1 H2O/D2O at 293 K.