Figure 2.

Configurations of M71 glomeruli in bulbs of conditional Nrp1 knockout mice. A, Triple-mutant mice n5247MCZ. B, Quadruple-mutant mice NMRCZ. The occurrence of configurations I–VI is listed per age. Red numbers indicate the most frequent configuration for a given age. Positions of labeled glomeruli in the various configurations are illustrated schematically with green dots, and in the case of NMRCZ, with orange dots for glomeruli that consist clearly of both RFP⁺ and GFP⁺ axons, and green dots for glomeruli formed predominantly by GFP⁺ axons. No glomeruli formed by only RFP⁺ axons were observed. The data are pooled from whole-mount images and coronal sections. Pairwise χ² tests with Bonferroni correction for multiple comparisons were performed on successive age pairs for both strains: n5247MCZ, PD0 vs. PD14 (*), PD14 vs. PD21 [not significant (n.s.)], PD21 vs. PD105 (***); NMCZ, PD7 vs. PD14 (n.s.), PD14 vs. PD21 (n.s.), PD21 vs. PD35 (n.s.), PD35 vs. PD45 (n.s.), PD45 vs. PD 70 (*). The pre–multiple-comparison sigma values are designated as *p < 0.05, **p < 0.01, and ***p < 0.001.