Figure 3.

Three-dimensional reconstructions of bulbs of PD21 gene-targeted mice expressing M71 with and without Nrp1. Serial block-face two-photon tomography was carried out to image the intrinsic fluorescence in four bulbs of four homozygous M71-IRES-tauYFP mice at PD21, and in six bulbs of six triple-mutant n5247MCZ mice at PD21. Each individual mouse is indicated with a distinct color. The gray outer area represents the surface of the glomerular layer, and the black inner area the regions of the bulb below the glomerular layer. A, Dorsal view, comparable to the view in Figure 1. The medial M71 glomeruli are poorly visible in this dorsal view, because they reside in a flat, medial domain of the bulb. B, Dorsomedial view. The ectopic anterior glomeruli reside in the rostral tip of the bulb. Both the medial and lateral glomeruli are visible here by making the glomerular layer transparent. The bulb is tilted slightly laterally to expose the medial glomeruli better. C, Close-ups of views oriented in such a way that the individual glomeruli are separated clearly: dorsal, anterior, and medial domains of the bulb.