Figure 4. Rapamycin reduces macrophage accumulation in irradiated lungs.
Lung tissue from C57BL/6NCr mice treated with 5×6 Gy of thoracic IR and control or rapamycin formulated diet was collected at 16 weeks after IR and processed for immunohistochemistry (n=3 per condition). The number of T-cells (A), neutrophils (B), and macrophages (C) detected per high power field (HPF, 63×) were compared between each treatment. DAB (brown) was used as the chromogen in each example with hematoxylin as the counterstain (left panels). Columns: mean, error bars: SD, brackets: p< 0.05 by ANOVA.