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. Author manuscript; available in PMC: 2016 Dec 3.
Published in final edited form as: Cell. 2015 Dec 3;163(6):1484–1499. doi: 10.1016/j.cell.2015.10.065

Figure 1. Proximity Mapping Of The Centrosome-Cilium Interface.

Figure 1

A. Schematic representation of the mammalian centrosome and centrosome/basal body-primary cilium interface. Distal and subdistal appendages are indicated in red and blue, respectively.

B. Bait proteins used in this study, grouped and color-coded according to primary localization. Note that CEP290 and OFD1 localize to both centriolar satellites and transition zone/centriole, respectively.

C. Mass spectrometry Dot Plot view of bait-bait interactions. Dot shading (grey – black gradient) indicates total number of spectral counts detected for each prey protein. Dot size indicates relative abundance of prey protein in each BioID analysis. Confidence levels for each bait-bait interaction according to SAINT (significance analysis of interactome; (Teo et al., 2014)) false discovery rate (FDR) are indicated by dot border (light grey <5% FDR; black <1% FDR). Green boxes, previously reported interaction; red box border, BioID bait-bait interaction validated here by co-IP. Four previously reported bait-bait interactions were verified by co-IP as controls (green box highlighted with red border). Baits not interacting with any other bait protein omitted for clarity.

D. Bait-bait PxIs detected in our study. Each node (color-coded circle) represents a unique bait protein associated with the indicated centrosome-cilium substructure. 70 previously reported (green edges), and 206 new (black edges) bait-bait interactions were detected. 30 bait-bait PxIs validated by co-IP highlighted in red. Edge thickness is proportional to peptide counts (maximum number of counts detected in a single MS analysis, or MaxSpec).