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. 2016 Jun 11;64(4):1105–1120. doi: 10.1002/hep.28637

Figure 4.

Figure 4

Survivin protects HCC cells from inflammatory TNFα‐mediated cell death. (A) Experimental scheme of etanercept treatment. Survivin was deleted in HCC‐bearing Mx‐cre; svv f/f at the age of 8 months. These mice were then treated with etanercept at 10 mg/kg body weight intraperitoneally twice a week. (B) Cell death in svv Δli* HCCs was dramatically reduced after etanercept treatment for 1 week as determined by TUNEL staining. (C) HCC development was analyzed in svv Δli* mice after etanercept treatment. HCCs were quantified. n = 3 for each group. * P < 0.05, t test. (D) Schematic of TNFα‐mediated synergistic lethal effect of senescence and apoptosis sensitization; Survivin depletion induced impaired mitosis and senescence in cancer cells, whereas infiltration of inflammatory cells and senescence‐associated TNFα triggered cell death in senescent and neighboring nonsenescent cancer cells lacking Survivin. Abbreviations: BMT, bone marrow transplantation; DAPI, 4′,6‐diamidino‐2‐phenylindole; PBS, phosphate‐buffered saline.