Table 1.
Top canonical pathways represented by identified changes in the synaptic proteomes in individual brain regions
| Top canonical pathways | Auditory cortex | Frontal cortex | Hippocampus | Striatum | ||||
|---|---|---|---|---|---|---|---|---|
| p‐value | n r/n tot | p‐value | n r/n tot | p‐value | n r/n tot | p‐value | n r/n tot | |
| Clathrin‐mediated endocytosis signaling | 3.31E‐03 | 4/185 | 9.11E‐08 | 11/185 | 6.31E‐03 | 6/185 | 1.58E‐06 | 9/185 |
| Axonal guidance signaling | 0.422 | 2/433 | 3.57E‐02 | 7/433 | 1.55E‐02 | 9/433 | – | – |
| Calcium signaling | 0.118 | 2/178 | 3.87E‐02 | 4/178 | 0.211 | 3/178 | 8.91E‐02 | 3/178 |
| Regulation of cellular mechanics by calpain protease | – | – | 0.335 | 1/57 | 1.66E‐03 | 4/57 | 0.288 | 1/57 |
| RhoA signaling | 0.333 | 1/122 | 0.216 | 2/122 | 1.35E‐05 | 8/122 | 3.63E‐02 | 3/122 |
| Notch signaling | – | – | 2.49E‐03 | 3/38 | 0.288 | 1/38 | 0.202 | 1/38 |
| Remodeling of epithelial adherens junctions | 2.14E‐02 | 2/68 | 8.97E‐06 | 6/68 | 3.16E‐03 | 4/68 | 7.08E‐04 | 4/68 |
| Glutamate receptor signaling | 0.173 | 1/57 | 0.335 | 1/57 | 1.66E‐03 | 4/57 | 0.288 | 1/57 |
| GABA receptor signaling | 2.09E‐02 | 2/67 | 1.16E‐04 | 5/67 | 0.119 | 2/67 | 6.76E‐04 | 4/67 |
| Dopamine receptor signaling | – | – | – | – | 0.502 | 1/78 | 1.20E‐03 | 4/78 |
| Synaptic long‐term potentiation | 5.89E‐02 | 2/119 | 0.208 | 2/119 | 2.19E‐02 | 4/119 | 0.157 | 2/119 |
For this analysis QIAGEN's Ingenuity® Pathway Analysis (IPA®, QIAGEN Redwood City, www.qiagen.com/ingenuity) was used: Selection criteria were minimum significance levels < 0.05 and at least three proteins per pathway in at least one brain region. n r/n tot – The table gives the number of regulated proteins identified in the proteomics screen (n r) in relation to the total number of proteins in the reference proteome of the defined Ingenuity® pathway (n tot). p‐values indicating significant overlaps are given in bold. For the analysis, the combined data of synaptic protein and phosphopeptide abundances (Tables S3 and S4) were used.