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Quantitative measurement of transfection efficiency. Real‐time RT‐PCR revealed that miR‐29b and Smad3 expression were significantly changed 24 h after transfection with miR‐29b mimics, pSUPER‐Smad3 or pSUPER‐Smad3‐1309. Compared with untreated controls, miR‐29b expression was significantly upregulated (A; *P < 0.05), while Smad3 was significantly downregulated (B; *P < 0.05) by both pSUPER‐Smad3 and pSUPER‐Smad3‐1309 transfection
