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. 2016 Jun 7;34(5):326–333. doi: 10.1002/cbf.3193

Table 2.

Primer sequences

Gene Primer Sequence
TGF‐β1 F 5′‐GTGACAGCAGGGATAACACACTG‐3′
R 5′‐CATGAATGGTGGCCAGGTC‐3′
Smad3 F 5′‐TCCTGGCTACCTGAGTGAAGA‐3′
R 5′‐GTTGGGAGACTGGACGAAAA‐3′
α‐SMA F 5′‐CATCATGCGTCTGGATCTGG‐3′
R 5′‐GGACAATCTCACGCTCAGCA‐3′
Col‐1 F 5′‐CCCGGGTTTCAGAGACAACTTC‐3′
R 5′‐TCCACATGCTTTATTCCAGCAATC‐3′
β‐Actin F 5′‐TGAAGTACCCCATCGAGCACG‐3′
R 5′‐CAAACATGATCTGGGTCATCTTCT‐3′
miR‐29b RT primer 5′‐GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACACTGATT‐3′
F 5′‐GCGCGCTAGCACCATTTG‐3′
R 5′‐CAGTGCAGGGTCCGAGGT‐3′
Primary miR‐29a/b1 exon 1 F 5′‐TACTGAACTGTCACGGCAGA‐3′
R 5′‐TGTAGTTAGCGACCTCTGCT‐3′
Primary miR‐29a/b1 exon 4 F 5′‐TTGCACCCTCACGACATGCT‐3′
R 5′‐TGACTCTCAGCAGGCCTCA‐3′
Primary miR‐29b2/c exon 1 F 5′‐ACTTCTTTAGGGGTGTGCGTA‐3′
R 5′‐ACCCATCTCCCTAGCATTCT‐3′
Primary miR‐29b2/c exon 6 F 5′‐TCAGACTTGCCACCTGGACT‐3′
R 5′‐AGTTGGCATGAGGCTTCGA‐3′
U6 RT primer 5′‐AACGCTTCACGAATTTGCGT‐3′
U6‐F 5′‐CTCGCTTCGGCAGCACA‐3′
U6‐R 5′‐AACGCTTCACGAATTTGCGT‐3′