TGF‐β1 |
F |
5′‐GTGACAGCAGGGATAACACACTG‐3′ |
R |
5′‐CATGAATGGTGGCCAGGTC‐3′ |
Smad3 |
F |
5′‐TCCTGGCTACCTGAGTGAAGA‐3′ |
R |
5′‐GTTGGGAGACTGGACGAAAA‐3′ |
α‐SMA |
F |
5′‐CATCATGCGTCTGGATCTGG‐3′ |
R |
5′‐GGACAATCTCACGCTCAGCA‐3′ |
Col‐1 |
F |
5′‐CCCGGGTTTCAGAGACAACTTC‐3′ |
R |
5′‐TCCACATGCTTTATTCCAGCAATC‐3′ |
β‐Actin |
F |
5′‐TGAAGTACCCCATCGAGCACG‐3′ |
R |
5′‐CAAACATGATCTGGGTCATCTTCT‐3′ |
miR‐29b |
RT primer |
5′‐GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACACTGATT‐3′ |
F |
5′‐GCGCGCTAGCACCATTTG‐3′ |
R |
5′‐CAGTGCAGGGTCCGAGGT‐3′ |
Primary miR‐29a/b1 exon 1 |
F |
5′‐TACTGAACTGTCACGGCAGA‐3′ |
R |
5′‐TGTAGTTAGCGACCTCTGCT‐3′ |
Primary miR‐29a/b1 exon 4 |
F |
5′‐TTGCACCCTCACGACATGCT‐3′ |
R |
5′‐TGACTCTCAGCAGGCCTCA‐3′ |
Primary miR‐29b2/c exon 1 |
F |
5′‐ACTTCTTTAGGGGTGTGCGTA‐3′ |
R |
5′‐ACCCATCTCCCTAGCATTCT‐3′ |
Primary miR‐29b2/c exon 6 |
F |
5′‐TCAGACTTGCCACCTGGACT‐3′ |
R |
5′‐AGTTGGCATGAGGCTTCGA‐3′ |
U6 |
RT primer |
5′‐AACGCTTCACGAATTTGCGT‐3′ |
U6‐F |
5′‐CTCGCTTCGGCAGCACA‐3′ |
U6‐R |
5′‐AACGCTTCACGAATTTGCGT‐3′ |