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. 2016 Nov 1;11(11):e0164308. doi: 10.1371/journal.pone.0164308

Fig 4. Representative zymograms revealing gelatinolytic activity in serum after incubation with APMA for different incubation times.

Fig 4

Serum from patients with ascending aortic aneurysms and healthy controls was incubated with 2 mM APMA for different times to exclude that active MMP-2 can be generated from serum MMP-2 after longer incubation times. Lane 1: Human full length MMP-2 after activation with 2mM APMA for 2 hours. Lane 2: Human full length MMP-2 as delivered. A. Serum from patients with ascending aortic aneurysms was incubated with 2mM APMA for 15–240 minutes. All lanes show bands corresponding to pro-MMP-2 and bands corresponding to intermediate MMP-2. No signals for active 65kDa MMP-2 became visible after prolonged incubation time with APMA. B. Serum from healthy controls was incubated with 2mM APMA for 15–240 minutes. Lanes show bands corresponding to pro-MMP-2 and intermediate MMP-2. No signals relatable to active MMP-2 appeared after prolonged incubation times with APMA. C. Serum from patients with ascending aortic aneurysms was incubated with 2mM APMA for 0,25–24 hours. All lanes show bands corresponding to pro-MMP-2 and intermediate MMP-2. No signals relatable to active MMP-2 were detectable.