Skip to main content
. 2016 Nov 1;12(11):e1006351. doi: 10.1371/journal.pgen.1006351

Fig 2. Loss of cilia results in aberrant production of GLI3 and GLI2 isoforms in the FNP.

Fig 2

(A) Western blot analysis of GLI3FL, GLI3R, GLI2FL and GLI2R from the FNP of e11.5 embryos (inset schematic, FNP indicated in red). Production of GLI3FL and GLI2FL is increased relative to wild-type embryos, whereas production of GLI3R and GLI2R in both Kif3afl/fl;Wnt1-Cre and Ift88fl/fl;Wnt1-Cre is decreased relative to wild-type FNPs. (B, C) Quantitative analysis of Western blot in (A) by ImageJ. The ratios for GLI3FL to GLI3R and GLI2FL to GLI2R are shown. (D) Nuclear fractionation from the FNP of e11.5 embryos shows aberrant levels of GLI3FL, GLI3R, GLI2FL and GLI2R were conserved in the nucleus in both Kif3afl/fl;Wnt1-Cre and Ift88fl/fl;Wnt1-Cre. α-Tubulin was used as the loading control for total cell lysate. GAPDH and Lamin A/C were used as the loading control for cytosolic and nuclear fraction, respectively. Inset schematics of facial prominences in A and D indicate FNP (red) was harvested for the experiment.