Table 1. Table of genotype and phenotype frequency for wild-type, Kif3afl/fl;Wnt1-Cre and Kif3afl/fl;Wnt1-Cre;Gli3Δ699/+.
| Facial phenotype analyzed [n (%)]‡ | |||||
|---|---|---|---|---|---|
| Genotype | Stage | n (%)* | Frontal widening | Bilateral cleft | Duplicated nasal septum |
| Control | e11.5 | 117 (73.12%) | 0 (0%) | NA | NA |
| Kif3afl/fl;Wnt1Cre | e11.5 | 22 (13.75%) | 22 (100%) | NA | NA |
| Kif3afl/fl;Wnt1-Cre;Gli3Δ699+/- | e11.5 | 21 (13.13%) | 0 (0%) | NA | NA |
| Control | e14.5 | 12 (54.55%) | 0 (0%) | 0 (0%) | 0 (0%) |
| Kif3afl/fl;Wnt1-Cre | e14.5 | 6 (27.27%) | 6 (100%) | 6(100%) | 6 (100%) |
| Kif3afl/fl;Wnt1-Cre; Gli3Δ699+/- | e14.5 | 4 (18.18%) | 0 (0%) | 0 (0%) | 0 (0%) |
Genotypes of control including Kif3afl/fl, Kif3afl/+, Kif3a+/+, Kif3afl/+;Wnt1Cre, Kif3afl/fl;Gli3d699+/-, Kif3afl/+;Gli3d699+/-, Kif3afl/+;Wnt1Cre;Gli3d699+/-.
*n, number of embryos analyzed; %, percentage relative to the number of total embryos (total embryos for e11.5 n = 160; for e14.5 n = 22).
‡n, number of embryos presenting phenotypes; %, percentage relative to the number of embryos analyzed.
Frontal widening is defined by a distance between nasal pits greater than 1.5 mm in e11.5 embryos and greater than 1.8 mm e14.5 embryos.
Bilateral clefting is defined by a patent area greater than 0.103mm2 in e14.5 embryos.