Fig 7. Effect of exogenous application of basolateral exosomes from LLC-PK1 cells on amiloride-sensitive transepithelial current measurement in mpkCCD cells.

Cells were cultured for 10 days to allow the formation of tight junctions and the measurement of resistances and voltages across confluent monolayers. Exosomes isolated from conditioned media present on the basolateral side of LLC-PK1 cells cultured on permeable transwell inserts were applied to the apical side of mpkCCD cell monolayers. Application of these exosomes resulted only in a modest decrease in transepithelial current over time. Each point represents the mean ± s.e. from 3 separate inserts containing cells (N = 3).