Fig 8. Effect of exosomes isolated from conditioned media on the apical side of LLC-PK1 cells on ENaC activity in Xenopus 2F3 cells.
(A): representative single-channel recording using the cell-attached configuration shows a decrease in ENaC activity after application of apical plasma membrane LLC-PK1 exosomes to the apical side of Xenopus 2F3 cells. The dashed lines denote open and closed levels (o, open and c, closed). The number of current levels represents the number of channels in the patch. The arrow indicates the time point at which the exosomes were applied to the cells. (B): summary line graph showing the open probability (Po) of ENaC decreased within 10 minutes of applying exosomes to the apical surface of Xenopus 2F3 cells. Each point represents the mean ± s.e. and the data shown are from 6 separate patches (N = 6); P = 0.07.