Fig 10. Effect of ENaC activity in freshly isolated split-open tubules from SV129 wild-type mice after application of apical plasma membrane LLC-PK1 exosomes.
(A) Representative single-channel recording showing a decrease in ENaC activity in native mouse collecting duct cells after application of exosomes isolated from conditioned media within the apical side of LLC-PK1 cells. The dashed lines denote open and closed levels (o, open and c, closed). The number of current levels represents the number of channels in the patch. The arrow indicates the time point at which the exosomes were applied. (B) summary line graph showing the open probability (Po) of ENaC decreased after applying exosomes isolated from proximal tubule cells. Each point represents the mean ± s.e. and the data shown are from 8 separate patches (N = 8). Two data points are similar and the lines overlap; *P<0.01.