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. 2016 Nov 1;12(11):e1005998. doi: 10.1371/journal.ppat.1005998

Fig 1. Generation of transgenic T. musculi and expression of GFP.

Fig 1

(A) Schematic depiction of expression vector pSSUGFP, utilising 5´ and 3´ regions to integrate into the 18S SSU rRNA gene locus, and both PFR and β-α tubulin intergenic regions to effect RNA processing of eGFP and blasticidin resistance genes. (B) Detection of eGFP expression by western blot. Lane 1: Wild type T. musculi; Lane 2: Parasites transformed with expression cassette in which eGFP was incorporated into the tubulin gene array (pTubGFP); Lane 3: Expression cassette with eGFP incorporated into the SSU rRNA locus (pSSUT7GFP) in a cell line with T7 RNA polymerase incorporated into the tubulin array (pT7polyNeo); Lane 4: Expression cassette with eGFP incorporated into the SSU rRNA locus alone (pSSUGFP). Molecular mass markers are shown in kDa. See S2 Fig for different constructs. (C) Detection of eGFP expression by fluorescence microscopy: eGFP incorporated into the SSU rRNA locus.