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. 2016 Nov 1;11(11):e0165929. doi: 10.1371/journal.pone.0165929

Table 1. Evolutionary analysis of Solanaceae PIF genes.

    PIF1a PIF1b PIF3 PIF4 PIF7a PIF7b PIF8a
Number of sequences 3 3 3 3 3 3 3
Number of codons 552 499 702 495 435 397 461
Mean dNa ± SD 0.02±0.01 0.05±0.03 0.02±0.01 0.01±0.01 0.01±0.01 0.06±0.04 0.02±0.01
Mean dSb ± SD 0.08±0.04 0.08±0.04 0.07±0.03 0.04±0.01 0.05±0.03 0.16±0.11 0.07±0.02
Mean Ncc ± SD 55.03±0.80 54.38±0.45 50.21±0.73 51.41±0.31 53.08±0.53 54.03±0.52 59.20±0.42
M0d ω0g 0.29 0.73 0.45 0.28 0.36 0.42 0.26
p0h 1.00 1.00 1.00 1.00 1.00 1.00 1.00
lnli -2752.11 -2668.30 -3471.89 -2250.83 -2047.17 -2327.67 -2241.83
M1ae ω0 0.29 0.00 0.00 0.00 0.36 0.00 0.19
p0 1.00 0.36 0.57 0.73 1.00 0.58 0.91
ω1j 1.00 1.00 1.00 1.00 1.00 1.00 1.00
p1k 0.00 0.64 0.43 0.27 0.00 0.42 0.09
lnl -2752.11 -2666.07 -3469.56 -2249.99 -2047.17 -2318.59 -2241.72
M2af ω0 0.29 0.00 0.00 0.00 0.36 0.14 0.19
p0 1.00 0.59 0.69 0.79 1.00 0.69 0.91
ω1 1.00 1.00 1.00 1.00 1.00 1.00 1.00
p1 0.00 0.00 0.00 0.17 0.00 0.29 0.06
ω2l 1.00 1.94 1.51 2.62 1.00 19.48 1.00
p2m 0.00 0.41 0.31 0.05 0.00 0.02 0.03
lnl -2752.11 -2664.59 -3469.21 -2249.89 -2047.17 -2313.73 -2241.72
2Δl (M1a-M0)n 0 4.44 4.66 1.7 0 18.15 ** 0.24
2Δl (M2a-M1a)o 0 2.98 0.7 0.19 0 9.72* 0

a dN a dN: non-synonymous distances and the corresponding standard deviation (SD)

b dS: synonymous distances and the corresponding standard deviation (SD)

c Nc: effective number of codons and the corresponding standard deviation (SD)

d M0: the null hypothesis, one-ratio model

e M1a: nearly neutral model

f M2a: positive selection model

g ω0: ω estimates for the codons under purifying selection

h p0: estimated proportion of codons under purifying selection

i lnl: log likelihood of model

j ω1: ω estimates for the codons under neutral evolution

k p1: estimated proportion of codons under neutral evolution

l ω2: ω estimates for codons under positive selection

m p2: estimated proportion of codons under positive selection

n 2Δl (M1a–M0): the likelihood ratio statistics (2Δl) is approximated by the χ2 distribution (degree of freedom = 1), null hypothesis (M0) rejected is highlighted in bold

o 2Δl (M2a-M1a): the likelihood ratio statistics (2Δl) is approximated by the χ2 distribution (degree of freedom = 2), M1a rejected is highlighted in bold. Single and double asterisk indicate P<0.01 and P<0.001, respectively.