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. Author manuscript; available in PMC: 2017 Oct 28.
Published in final edited form as: ACS Sens. 2016 Oct 12;1(10):1208–1212. doi: 10.1021/acssensors.6b00433

Figure 3.

Figure 3

A) A subset of inhibitory natural product hits were rescreened in Ins-GLuc MIN6 cells by treating 24 h (1 µg/ml), washing and preincubation in KRBH with 200 µM diazoxide (Dz), followed by stimulation with 35 mM KCl and 20 mM glucose for 1 h. Bar graph represents luciferase activity in supernatants ± S.D. B) Fraction SNF009-8 was selected for further subfractionation by reversed-phase HPLC yielding 10 isolates. C) The subfractions were assessed phenotypically for activity as in (A). D) To probe acute effects of the subfractions, Ins-GLuc MIN6 cells were pretreated for 1 h with SNF009-8-7 and –CA3 (1 µg/ml) in KRBH before 1 h stimulations in the diazoxide paradigm. E) MIN6 β cell calcium influx assays were performed on cells pretreated overnight with SNF009-8-7. Cells were loaded with Fura-2-AM (5 µM), stimulated with 35 mM KCl, and monitored for 2 min.