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. Author manuscript; available in PMC: 2017 Oct 10.
Published in final edited form as: J Biotechnol. 2016 Apr 12;235:121–131. doi: 10.1016/j.jbiotec.2016.04.023

Figure 6. Visualization of clustered centromeres in P. pastoris by confocal microscopy.

Figure 6

This strain expressed DsRed-HDEL to label the endoplasmic reticulum in red. The ring visible in each cell is the nuclear envelope. In addition, the strain expressed Cse4-GFP to label centromeres in green. The merged image shows the two fluorescence signals overlaid on a transmitted light image of the cells. A cluster of centromeres is visible at the nuclear periphery in each cell. Scale bar, 2 μm.