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. Author manuscript; available in PMC: 2017 Oct 10.
Published in final edited form as: J Biotechnol. 2016 Apr 12;235:121–131. doi: 10.1016/j.jbiotec.2016.04.023

Table 5. Putative open reading frames identified on the 13.1 kbp and 9.5 kbp plasmids of P. pastoris.

All protein sequences were analyzed using blastp (protein-protein blast) against the non-redundant protein database. The protein entries showing the highest sequence identity to the query are summarized. No homologues of the gamma toxin subunit or the antitoxin gene were identified on the two P. pastoris killer plasmids.

3.1 kbp plasmid Description Organism and sequence identity
ORF1 DNA-polymerase Debaryomyces hansenii 67%
ORF2 mRNA capping enzyme Pichia etchellsii 49%
ORF3 Helicase K. lactis 59%
ORF4 DNA binding protein Millerozyma acacia 54%
ORF5 RNA-polymerase K. lactis 57%
ORF6 RNA-polymerase subunit M. acacia 41%
ORF7 Killer toxin protein K. lactis 50%
ORF8 terminal recognition factor M. acacia 58%
9.5 kbp plasmid
ORF1 DNA-polymerase D. hansenii 55%
ORF2 Hypothetical protein no similarity
ORF3 Hypothetical protein no similarity
ORF4 Killer toxin protein K. lactis 46%
ORF5 Hypothetical protein no similarity
ORF6 Hypothetical protein no similarity