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. Author manuscript; available in PMC: 2017 Oct 20.
Published in final edited form as: Mol Cell. 2016 Oct 13;64(2):236–250. doi: 10.1016/j.molcel.2016.09.009

Figure 1. The Fas/FADD Complex, FADDDED and ASCPYD Promote Casp-8tDED Filament Formation.

Figure 1

(A) Domain composition and interaction hierarchy of Casp-8 activation in the death receptor pathway and the inflammasome pathway.

(B) A gel filtration profile of GFP-Casp-8tDED purification. An SDS-PAGE of the major peaks is shown. Peak 1 at the void position contained the target protein.

(C) An electron micrograph of GFP-Casp-8tDED filaments.

(D) Fluorescence polarization (FP) assay of FasDD/FADD, FADD and ASCPYD nucleated Casp-8tDED filament formation.

(E) Streptavidin-gold labeling of the FasDD/FADD-biotin/Casp-8tDED filaments, showing the end localization of the FasDD/FADD-biotin complex.

(F, G) Nucleation of Casp-8tDED filament formation by the FasDD/FADD complex (F) and FADDDED (G). Calculated apparent dissociation constants (Kapp), with fitting errors, are shown above the plots.

See also Figure S1.