Skip to main content
. Author manuscript; available in PMC: 2017 Oct 20.
Published in final edited form as: Mol Cell. 2016 Oct 13;64(2):236–250. doi: 10.1016/j.molcel.2016.09.009

Figure 5. Filament Formation-Defective Mutants of Casp-8 Compromised Death Receptor- and Inflammasome-Mediated Signaling.

Figure 5

(A) Cell death in Casp-8 deficient Jurkat cell line I9.2C reconstituted with WT and mutant Casp-8 induced by isoleucine zipper-fused FasL (FasL-LZ).

(B) Histogram of cell death in I9.2C reconstituted with WT and mutant Casp-8 in the presence of 50 ng/ml Fas-LZ.

(C) Analysis of ASC-mediated Casp-8 speck formation using flow cytometry. All Casp-8-Myc constructs contain the C360S mutation in addition to the stated mutations. WT refers to Casp-8 C360S without any mutation in the tDED. Cells were stained with Myc and ASC antibodies and analyzed by flow cytometry. Cells with an elevated peak height to area ratio for Casp-8 show concentration of Casp-8 on the ASC speck (Sester et al., 2015) and are boxed.

(D) Casp-8 speck formation in cells transfected with the indicated Casp-8 plasmids, with or without ASC. Mean and range of duplicate transfections in a representative experiment are shown.

(E) ASC speck formation in cells transfected with ASC and the indicated Casp-8 plasmids. Mean and range of duplicate transfections in a representative experiment are shown.

See also Figure S5.