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. Author manuscript; available in PMC: 2016 Nov 1.
Published in final edited form as: Cell Rep. 2016 Oct 25;17(5):1207–1216. doi: 10.1016/j.celrep.2016.10.003

Figure 2. The IRE1 target, XBP1, is required for lipid accumulation and starvation survival in the midgut and is sufficient to rescue IRE1 depletion.

Figure 2

A. Oil Red O staining of midguts. Flies fed for 10-12 days on AL or DR (−/+RU486 for XBP1 depletion; the black line denotes the anterior midgut area where lipids accumulate when fed a DR diet. A’. Quantification of ORO signal from (A) in the anterior midgut. n=10 flies/condition. B. Starvation resistance upon XBP1 depletion in midgut enterocytes, after 10-12 days of feeding in differentiated food. n=100 flies/condition, from 4 independent crosses. C. Oil Red O lipid staining of midguts from flies fed on DR shows rescue of lipid stores after over-expression of spliced XBP1 (UAS-XBP1s) in an IRE1 depletion background. Scale bar 200 µm, n=10-12 flies/condition. C’. Quantification of ORO signal from (C), in the anterior midgut. n=9-10 flies/condition. D. Spliced XBP1 (UAS-XBP1s) was over-expressed in a 5966GS-GAL4;IRE1RNAi background and is sufficient to rescue the starvation sensitivity upon IRE1 depletion.

In all panels, error bars show SEM; statistical significance and interaction between Diet and RNAi, was determined by two-way ANOVA with Bonferonni’s post hoc test. See also related Figure S2.