Figure 2.

Ziram causes alterations in zebrafish (ZF) motor behavior. Movement was tracked for larval ZF at 7 days post-fertilization (dpf) (n = 24). Distances > 2 mm were tracked under an alternating light (yellow)/dark (gray) cycle (A). A 24.7% decrease (p < 0.05) in distance traveled during periods of dark was observed in ziram-treated ZF relative to vehicle-treated ZF (B), but no significant difference was observed during periods of light (C). A similar pattern of swimming, less in the dark but no difference in the light, was measured when ZF were treated with the dopamine antagonist haloperidol (B,C). The dopamine agonist apomorphine (Apo) increased swimming to a similar degree in the ziram-treated and control ZF, suggesting that the postsynaptic dopaminergic system remained intact. No significant difference in motor behavior was observed for ziram + apomorphine versus apomorphine alone for light or dark conditions. All treatment groups were compared with vehicle controls for statistical analysis. *, p < 0.05; ***, p < 0.001; ****p < 0.0001; one-way analysis of variance (ANOVA).