Fig. 3.

Influence of MCPIP-1 on E. coli-induced expression of IL-8 in HeLa and Caco-2 cell lines. a, b MCPIP-1 was silenced in HeLa cells using specific siRNA or shRNA sequences; scrambled nonselective sequences were used as NCs. The cells were subsequently infected with E. coli. After the indicated times, culture media were collected and the level of IL-8 was estimated by ELISA. c MCPIP-1 was silenced in Caco-2 cells using three specific siRNAs; scrambled nonselective sequences were used as NCs. The level of MCPIP-1 protein after transfection was determined by Western blotting (above). The IL-8 level was estimated in culture media of noninfected and E. coli-infected cells (24 h postinfection). Levels of secreted IL-8 from three independent experiments are presented as absolute values (pg/ml) ± SD. * p < 0.05; ** p < 0.01. n.s. = Not significant.