Fig. 5.

Effect of MCPIP-1 overexpression in HeLa cells on constitutive and E. coli-induced IL-8 levels. a Levels of MCPIP-1 in HeLa cells after transduction with empty vector or expression plasmid-harboring WT MCPIP-1 was verified by Western blotting (above). Using conditioned media, IL-8 and IL-6 protein levels were determined by ELISA, and the MCP-1 level by cytometric bead array. Bars represent the mean concentrations of secreted cytokines ± SD. b Control and MCPIP-1-overexpressing cells were infected with E. coli. At the indicated times postinfection, media were harvested and levels of secreted IL-8 were estimated by ELISA. Data represent mean values from three or four independent experiments ± SD. * p < 0.05; ** p < 0.01. n.s. = Not significant.