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MCPIP-1 directly regulates the stability of IL-8 mRNA. a Purity of recombinant MCPIP-1 protein (WT and D141N) used in the experiments, documented by SDS PAGE. b Degradation of IL-8 transcript by recombinant MCPIP-1 protein (WT), estimated by in vitro RNA cleavage assay. c Interaction of recombinant MCPIP-1 lacking RNase activity (D141N) with the IL-8 transcript (CDS + 3′UTR), estimated by EMSA. Results are representative of three independent experiments.
