Table 2.
PCR primers used for the detection of STEC virulence or adherence genes (Bai et al., 2016).
| Target | Primer forward/Primer reverse (5′-3′) | Amplicon size (bp) | Annealing temperature (°C) |
|---|---|---|---|
| stx1 | AAATCGCCATTCGTTGACTACTTCT/ | 370a | 58 |
| TGCCATTCTGGCAACTCGCGATGCA | |||
| stx2 | CAGTCGTCACTCACTGGTTTCATCA/ | 283a | 58 |
| GGATATTCTCCCCACTCTGACACC | |||
| stx2 | ATGAAGTGTATATTATTTAAATGG/ | 1260b | 55 |
| TCAGTCATTATTAAACTGCAC | |||
| eae | TCAATGCAGTTCCGTTATCAGTT/ | 482 | 58 |
| GTAAAGTCCGTTACCCCAACCTG | |||
| efa1 | GAGACTGCCAGAGAAAG/ | 479 | 51 |
| GGTATTGTTGCATGTTCAG | |||
| saa | CGTGATGAACAGGCTATTGC/ | 119 | 52 |
| ATGGACATGCCTGTGGCAAC | |||
| paa | ATGAGGAAACATAATGGCAGG/ | 350 | 60 |
| TCTGGTCAGGTCGTCAATAC | |||
| astA | CCATCAACACAGTATATCCGA/ | 111 | 55 |
| GGTCGCGAGTGACGGCTTTGT | |||
| ehxA | GGTGCAGCAGAAAAAGTTGTAG/ | 1551 | 57 |
| TCTCGCCTGATAGTGTTTGGTA | |||
| toxB | ATACCTACCTGCTCTGGATTGA/ | 602 | 55 |
| TTCTTACCTGATCTGATGCAGC |
a
Primers for detection of stx1 and stx2 using duplex PCR.
b
Primers used for amplifying and sequencing the full length of stx2.