FIGURE 4.

Purkinje cell torpedoes unlikely to be presynaptic terminals. (A) Sample image of Purkinje cell with torpedo (green) that is co-stained with the presynaptic inhibitory terminal marker VGAT (red). Inset shows that torpedo does not label for VGAT. Scale bar for left image, 20 μm; scale bar for insets to right, 5 μm. (B) The vast majority of torpedoes at both P11 and P30 are not positive for VGAT (white bars) with only a subset having any VGAT labeling nearby (VGAT+, blue bars), suggesting that they are unlikely to be presynaptic terminals. Number of torpedoes (N) counted is indicated in bars. Significance determined by Mann–Whitney U test, ns P > 0.05.