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. 2016 Nov 2;10:248. doi: 10.3389/fncel.2016.00248

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Copyright © 2016 Ljungberg, Lang-Ouellette, Yang, Jayabal, Quilez and Watt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Most developmental torpedoes are myelinated. (A) Sample image showing Purkinje cells and axons (green) as well as myelin basic protein (MBP; red). Scale bar, 20 μm. (B) The vast majority of torpedoes at both P11 and P30 are positive for MBP (white bars), with only a fraction negative for MBP (red). Number of torpedoes (N) is indicated in or above each bar. (C) Successive z-stack images (z_1-3) of region highlighted in panel (A), highlighting that myelin appears to fully wrap around Purkinje axon developmental torpedo. Scale bar, 5 μm. Significance determined by Mann–Whitney U test, ns P > 0.05.