Table 1.
Gene name | Primer sequence | Annealing temp. (°C) | Product size (bp) |
---|---|---|---|
Actin | Forward primer 5′ CTTGCACCTAGCAGCATGAA 3′ | 52 | 154 |
Reverse primer 5′ GGACAATGGATGGACCTGAC 3′ | |||
CS | Forward primer 5′ TGGGACAGAGCTCTTGGACT 3′ | 51 | 140 |
Reverse primer 5′ TCAGTGTGGAAGGAACACCA 3′ | |||
SUCLG1 | Forward primer 5′ ATTATGCCGGGTTACATCCA 3′ | 49 | 141 |
Reverse primer 5′ AAAAGGATCCCCACCAATTC 3′ | |||
SDH | Forward primer 5′ GTGGTCAGGCCTATCGTTGT 3′ | 54 | 154 |
Reverse primer 5′ CCCTGGCAAGTACCATCACT 3′ | |||
FH | Forward primer 5′ CTCTCCACCATCTCGTCTCC 3′ | 49 | 141 |
Reverse primer 5′ CCCTGAACGAGGTCGAATAA 3′ | |||
MS | Forward primer 5′ GGGCATGTGAGGTACGCTAT 3′ | 52 | 123 |
Reverse primer 5′ AGAGGCACAAACCCATTCAC 3′ | |||
CHLASE | Forward primer 5′ GAATATCCGGTGGTGATGCT 3′ | 49 | 161 |
Reverse primer 5′ TCCGCCGTTGATTTTATCTC 3′ | |||
PSY | Forward primer 5′ TGGGTTGGTAAGGGCTGTAG 3′ | 51 | 155 |
Reverse primer 5′ CGCTCGAAGACACAACACTC 3′ | |||
CHS | Forward primer 5′ CAAGGCGGAGAAGATGAGAG 3′ | 54 | 113 |
Reverse primer 5′ CATCTTCCGCAGACTTCCTC 3′ | |||
PAL | Forward primer 5′ AAACTCCGTCAACGACAACC 3′ | 54 | 142 |
Reverse primer 5′ AGCGAACATGAGCTTCCCTA 3′ |
CS, citrate synthase; SUCLG1, succinyl Co-A ligase; SDH, succinate dehydrogenase; FH, fumarate hydratase; MS, malate synthase; CHLASE, chlorophyllase; PSY, phytoene synthase; CHS, chalcone synthase; and PAL, phenylalanine ammonialyase.