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. 2016 Nov 2;7:1569. doi: 10.3389/fpls.2016.01569

Table 1.

Primer sequences used for gene expression analysis using qRT-PCR.

Gene name Primer sequence Annealing temp. (°C) Product size (bp)
Actin Forward primer 5′ CTTGCACCTAGCAGCATGAA 3′ 52 154
Reverse primer 5′ GGACAATGGATGGACCTGAC 3′
CS Forward primer 5′ TGGGACAGAGCTCTTGGACT 3′ 51 140
Reverse primer 5′ TCAGTGTGGAAGGAACACCA 3′
SUCLG1 Forward primer 5′ ATTATGCCGGGTTACATCCA 3′ 49 141
Reverse primer 5′ AAAAGGATCCCCACCAATTC 3′
SDH Forward primer 5′ GTGGTCAGGCCTATCGTTGT 3′ 54 154
Reverse primer 5′ CCCTGGCAAGTACCATCACT 3′
FH Forward primer 5′ CTCTCCACCATCTCGTCTCC 3′ 49 141
Reverse primer 5′ CCCTGAACGAGGTCGAATAA 3′
MS Forward primer 5′ GGGCATGTGAGGTACGCTAT 3′ 52 123
Reverse primer 5′ AGAGGCACAAACCCATTCAC 3′
CHLASE Forward primer 5′ GAATATCCGGTGGTGATGCT 3′ 49 161
Reverse primer 5′ TCCGCCGTTGATTTTATCTC 3′
PSY Forward primer 5′ TGGGTTGGTAAGGGCTGTAG 3′ 51 155
Reverse primer 5′ CGCTCGAAGACACAACACTC 3′
CHS Forward primer 5′ CAAGGCGGAGAAGATGAGAG 3′ 54 113
Reverse primer 5′ CATCTTCCGCAGACTTCCTC 3′
PAL Forward primer 5′ AAACTCCGTCAACGACAACC 3′ 54 142
Reverse primer 5′ AGCGAACATGAGCTTCCCTA 3′

CS, citrate synthase; SUCLG1, succinyl Co-A ligase; SDH, succinate dehydrogenase; FH, fumarate hydratase; MS, malate synthase; CHLASE, chlorophyllase; PSY, phytoene synthase; CHS, chalcone synthase; and PAL, phenylalanine ammonialyase.