FIG 2 .

GAS MVs carry classically secreted and anchorless virulence proteins. (A) Protein profiles of ISS3348 whole-cell extract (WC), cytoplasm (C), membrane (M), secreted fraction prior to ultracentrifugation (S1), MV, and secreted fraction after centrifugation (S2). (B) Western blot of virulence-associated proteins identified in MVs. The samples in each lane are the same as in panel A. (C) Swiss-Prot classification of nano-LC-MS/MS-identified MV proteins. (D) KEGG categories enriched in MV proteins. (E) Silver-stained 2D protein profile and respective 2D Western blot of ISS3348 MV proteins probed with ARF patient antisera and anti-human IgG-Fc-horseradish peroxidase (HRP).