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. 2015 Jul 28;16(4):045003. doi: 10.1088/1468-6996/16/4/045003

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© 2015 National Institute for Materials Science

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Figure 7. — Sagittal sections of porcine corneas. (a), (b) The corneal endothelium can be seen as a monolayer of squamous cells at the posterior corneal side before de-endothelialization. (c), (d) After removal of the porcine endothelium the bare Descemet’s membrane is visible. (e), (f) One day after transfer of HCEC-sheets onto de-endothelialized corneas the cells appeared attached to Descemet’s membrane as a confluent monolayer. Positive immunohistochemical staining against ZO-1 (g) and Na⁺/K⁺-ATPase α1 (h) of transferred HCEC-sheets shown in green (Alexa Fluor^®488), nuclei are displayed in blue (DAPI). n = 3.