Table 1.

Primary and secondary antibodies; ICC—immunocytochemistry, IHC—immunohistochemistry.

Antigen	Host	Clone	Supplier	Dilution; incubation time
Paxillin	Mouse	349/paxillin	BD Transduction Laboratories, Heidelberg, Germany	1:100; 60 min
Fibronectin	Rabbit	Polyclonal	Biomol, Rockland Immunochemicals, Hamburg, Germany	1:200; 60 min
Collagen Type IV	Goat	Polyclonal	Biozol, Southern Biotech, Eching, Germany	1:20; overnight
Laminin	Rabbit	Polyclonal	Sigma-Aldrich, Munich Germany	1:20; overnight
ZO-1	Mouse	1/ZO-1	BD Transduction Laboratories, Heidelberg, Germany	ICC: 1:100; 60 min; IHC: 1:400; overnight
Na+/K+-ATPase α1	Mouse	464.6	Abcam, Cambridge, UK	ICC: 1:10; overnight; IHC: 1:400; overnight
Alexa Fluor®488 anti-mouse IgG	Goat	Polyclonal	Life Technologies, Molecular Probes, Darmstadt, Germany	1:200; 45 min
Alexa Fluor®488 anti-rabbit IgG	Goat	Polyclonal	Life Technologies, Molecular Probes, Darmstadt, Germany	1:200; 45 min
Alexa Fluor®488 anti-goat IgG	Donkey	Polyclonal	Life Technologies, Molecular Probes, Darmstadt, Germany	1:200; 45 min