Fig. 5.
Changes of mass-specific Cdint in WT and mutants over 2 h on CdCl2-free NGM plates after pre-incubation on CdCl2-containing plates for 24 h. After 24-h incubations at 2 mmol/L of CdCl2, changes in mass-specific Cdint in WT and mutants on CdCl2-free NGM plates (mean ± SE; per mean: n = 3-16 tested groups with several hundred worms each) were evaluated with regard to the initial Cdint values (at 0 h; dashed lines), absolute decreases in Cdint within the first half hour (Cd release; Δ values), and Cdint values over the 2-h measurement period. The initial Cdint values of (B) WT and (F) hmt-1Δ differed significantly (dark red letter*, P = 0.03). Cd release differed significantly between (A) mrp-1Δ and (B) WT (dark red letter*, P = 0.04) as well as between (B) WT and (F) hmt-1Δ (dark red letter*, P = 0.02). The Cdint values over 2 h differed significantly (vertical lines and P values) between (A) mrp-1Δ and (C-F) mrp-4Δ, pcs-1Δ, pgp-1Δ and hmt-1Δ; between (B) WT and (E-F) pgp-1Δ and hmt-1Δ; and between (C-E) mrp-4Δ, pcs-1Δ, pgp-1Δ and (F) hmt-1Δ. Gray areas show differences to the Cdint values of mrp-1Δ. Statistical significance was tested by two-way ANOVA and subsequent SNK analyses (Cdint values over 2 h) or t-tests, with dark red letters indicating statistical significance.