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. 2016 Aug 24;35(21):2285–2300. doi: 10.15252/embj.201593103

Figure 7. Rescue of differentiation defects of trisomic ES cells by extracellular factors.

Figure 7

  1. Morphology of EBs grown for 8 days derived from individual or mixed ES cell lines. Hematoxylin and eosin staining. Scale bar, 100 μm.
  2. Quantification of Nanog expression in trisomic, wild‐type, and mixed ES cell‐derived EBs cultured for 8 days. Error bars, ± SD. n = 3. **P < 0.01.
  3. The morphology of EBs at day 10 of differentiation. M15 was the normal EB formation condition and was used as a control. Black arrows indicate the cystic structures. Scale bar, 200 μm.
  4. Box plots of EB diameter at day 10 of EB formation under M15, CM, and M15 supplemented with Bmp4 conditions. Each box plot was plotted for more than 10 EBs. Boxes indicate the 25th to 75th percentiles and the central bars represent the median. The ends of the whiskers represent the maximum and minimum values. EBs cultured under CM or Bmp4 conditions were compared to EBs of the same cell line cultured under M15 conditions. *P < 0.05, **P < 0.01.

Source data are available online for this figure.