Skip to main content
. 2016 Sep 16;35(21):2315–2331. doi: 10.15252/embj.201593454

Figure EV1. Validation of the evi1 morpholino oligonucleotides.

Figure EV1

  • A
    Schematic representation of protein domains of the transcription factor Evi1. Blue boxes represent zinc finger motifs in the Evi1 protein. Other functional domains are indicated.
  • B
    The evi1 splice donor MO, depicted by red crosses, target either the 3rd (MO1) or the 6th (MO2) zinc finger in the first zinc finger domain.
  • C
    RT–PCR of evi1 in embryos injected with evi1 MO1 or MO2 indicates splice modification. Corresponding primer pairs are shown. Expected wt bands for MO1 389 bp, in evi1 morphants 2,526 bp. For MO2: expected wt bands 1,100 bp, 5,695 bp for evi1 morphants.
  • D
    No pooling was observed by o‐dianisidine staining in evi1 morphants at 38 hpf.
  • E, E′
    Co‐injection of capped evi1 mRNA (E) or UAS:mEvi1 (in Tg(fli.1:Gal4FF;UAS:RFP) embryos, E') together with the evi1 MO rescues the HSC phenotype, shown by restored runx1/c‐myb expression in the VDA, marked with black arrowheads. Numbers indicate the amount of embryos with the respective phenotype/total number of embryos analyzed in each experiment. Arrows indicate up‐ or downregulation of runx1/c‐myb in each condition. Lateral views are shown, anterior to the left, dorsal up.