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. 2016 Sep 16;35(21):2315–2331. doi: 10.15252/embj.201593454

Figure 6. evi1 regulates Notch independently of the Vegf pathway and can compensate for Vegf inhibition.

Figure 6

  • A
    WISH of dll4 (upper) and notch1b (lower) in control‐injected embryos (left), evi1 morphants (middle), and evi1 morphant transgenic Tg(Hsp70l:vegfaa; myl7:eGFP) embryos at 32 hpf after heat‐shock induction at 20 hpf (right).
  • B, C
    WISH of runx1/c‐myb (B) or notch1b (C) after treatment with DMSO (left), the VEGF receptor inhibitor SU5461 (middle), and, respectively, SU5461 after injection of UAS:mEvi1 (inducing endothelial evi1 expression) in Tg(fli.1:Gal4FF;UAS:RFP) embryos (right).
Data information: Two or more biological replicates are shown for each experiment. Lateral views are shown, anterior to the left, dorsal up. Numbers indicate the amount of embryos with the respective phenotype/total number of embryos analyzed in each experiment. Arrows indicate up‐ or downregulation of the respective gene in each condition. For each analyzed gene, quantitation of results is shown, displaying the percentages of embryos with normal vs. decreased gene expression for each condition. A Fisher's exact test was applied to calculate statistical significance (*P < 0.05, **P < 0.01, ***P < 0.001).